Octet® Regeneration Buffer Kit

The Octet® Regeneration Buffer Kit provides a set of four ready-to-use buffers for quick screening of different regeneration conditions for various Octet® biosensors and a wide range of ligands and analytes. Regeneration allows for biosensor re-use and provides a cost-effective solution for analyzing a large number of samples or ligand-analyte pairs in sequence.Typically, regeneration can be performed by dipping biosensors into the regeneration buffer for a few seconds, followed by a dip in the assay buffer. These steps should be repeated 3-5 times in sequence to fully remove bound antibody or the interaction complex.Since different modes of interaction exist between ligands on the biosensor surface and target analytes, determining the optimal regeneration conditions that disrupt these interactions is essential and should be done for each application experimentally. This kit enables users to quickly screen multiple buffers and develop regeneration conditions specific to their needs.