Sartobind® Phenyl 96-Well Plates

Tech Support Documents
Sartobind® Phenyl 96-Well Plates
 

Sartobind® Phenyl accomplishes polishing and capturing ten times faster than do conventional HIC resins. The membrane shows comparable, high binding capacity, without size exclusion effects, for proteins in a range of 20 mg mAb/mL membrane, as well as excellent resolution and impurity reduction.

Pack Size
Item No.: 
99HICP42GC-----D
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

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Hydrophobic interaction chromatography (HIC) separates and purifies biomolecules based on differences in their hydrophobicity. The phenyl membrane adsorber follows the same rules known from the conventional hydrophobic interaction chromatography. Due to the large pore size, membrane adsorbers show excellent flow properties. There is almost no diffusion limitation of mass transport compared with conventional bead chromatography. Buffers with high concentrations of salt promote the adsorption of proteins on the hydrophobic membrane matrix. Proteins are eluted by decreasing the salt concentration in the elution buffer.

The salt dependence of the membrane is comparable to that of columns with ammonium sulfate, sodium chloride and sodium citrate.

Applications:

HIC is a powerful method for differentiating target protein and hydrophobic contaminants. Large proteins bind more strongly than do small ones and aggregates even considerably better than monomers due to multipoint attachment.

Buffer with a high ionic strength, usually ammonium sulfate, is initially added to the protein solution. Salt ions in this solution reduce the solvation of solutes in the sample. As solvation decreases, hydrophobic regions that become exposed are adsorbed by the hydrophobic membrane matrix. In the HIC membrane, flexible cellulose is used as a backbone for the phenyl ligand. The membrane can be regenerated by using either water or alcohol.

The HIC membrane is used for the removal of:

  • Aggregates
  • Host cell proteins
  • Viruses
  • Endotoxins
  • Lipids, dyes, detergents and anti foam agents
  • Leached chromatography ligands

The Phenyl membrane can be used also for binding large proteins such as:

  • Monoclonal antibodies
  • Conjugated vaccines, viruses and phages
  • Oligonucleotides

Benefits:

HIC membranes offer the following advantages over conventional HIC columns:

  • Excellent reduction of aggregates
  • 85 % buffer savings
  • 75 % of time saved

Easy to use:

  • Plug and play devices from 0.08 ml to 5 l
  • Can be used like a filter
  • Flow rate up to 5 bed volumes/min
  • Re-usable >100 cycles or disposable

Applications

  • Application
    Screening

Connections (Physical)

  • Connector(s)
    N/A

Dimensions

  • Bed Height
    0.8 mm
  • Dimensions WxDxH
    128 × 85.5 × 25 mm
  • Membrane Area
    0.7 cm²

Features

  • Reusable
    No

Materials of Construction

  • Housing Material
    Polypropylene (PP)
  • Material 8-strip
    Polypropylene (PP)
  • Material Holding Frame
    Polystyrene
  • Materials of Construction
    Polyester
  • Membrane Material
    Stabilized reinforced cellulose

Operating Conditions

  • Maximum Operating Pressure
    35 kPa
  • pH Stability
    pH 2 – 14 (short term)

Physicochemical Information

  • Bed Volume
    19 µL/well
  • Ligand
    Phenyl
  • Ligand Density
    3 µeq/cm²
  • Pore Size
    3 – 5 µm
  • Reference Protein
    Gamma globulin from bovine blood
  • Typical Dynamic Binding Capacity 10% per unit
    0.28 mg/well

Product Information

  • Brand
    Sartobind® Phenyl
  • Membrane Adsorber Format
    96-Well Plates
  • Membrane Type
    Hydrophobic interaction chromatography membrane (HIC)
  • Pack Size
    10
  • Type
    Membrane adsorber (96-well plate)
  • What you receive
    • 2 plates
    • 4 deep well collection plate 2 ml
    • 1 operating instruction
  • Vivawell Vac96 Vacuum Manifold
    Vivawell Vac96 Vacuum Manifold
    Item no.
    VW96VAC01

    The Vac96 vacuum manifold has been designed specifically for use with Sartobind® 96 multiwell plates. The long drip nozzles on the outlet eliminate cross-talk between flow channels.