MCDB 153 medium is a modification of Ham's nutrient mixture F-12 designed for the growth of non-transformed cell types in a serum-free formulation
Modified as follows
WithL-Glutamine
Phenol RedSodium Bicarbonate
Sodium Pyruvate
With hydrocortisone, T3, testosterone, insulin, transferrin and sodium selenite
MEM-NEAAGlucose