The 4Cell® CHO Feed TCX7D is a chemically defined, animal component-free cell culture feed supplement. It is developed for the use as feeding solution in large scale recombinant biopharmaceutical protein production. The feed supplement contains highly concentrated nutrients to increase the productivity of CHO cells. It does not contain lipids, hydrolysates, or growth factors. 4Cell® CHO Feed TCX7D supports superior cell growth and production of recombinant proteins and antibodies in suspension culture by maintaining and extending the production capability of CHO cultures compared to batch process. Consumed substances like vitamins and amino acids are replenished to increase the protein yield by process extension. 4Cell® CHO Feed TCX7D is especially well suited for CHO GS cell lines but can be used for other CHO cell lines as well when L-glutamine is supplemented.