CIMmultus® QA 8 mL Monolithic Column (2 μm)

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CIMmultus® QA 8 mL Monolithic Column (2 μm)
 

This high-performance ion exchange column is primarily intended for fast and efficient purification of biomolecules such as viruses, adeno-associated viruses (AAV), and virus-like particles (VLP) through their ionic interaction with the monolith.

Item No.: 
411.5113-2
Availability: 
Piece
1

*Custom/bulk order quotes are provided within 72 hours of request.

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CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.

CIMmultus® columns can be used as single-use disposable or multi-use columns. They are available in a variety of volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.

CIMmultus® QA is a high-performance ion exchange column primarily intended for fast and efficient purification of biomolecules such as viruses, adeno-associated viruses (AAV), and virus-like particles (VLP) through their ionic interaction with the monolith.

QA (quaternary amine) features:

  • Strong anion exchanger
  • Working range, pH 2–13
  • Binds molecules with a predominantly negative charge, repels molecules with a predominantly positive charge
  • Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
  • High capacity for virus particles (AAV), IgM, exosomes
  • High efficiency removal of endotoxin and DNA removal
  • Outstanding for preparative applications or analytical fingerprinting of process samples
  • Sanitizable with 1 M NaOH

Benefits:

  • Very fast processes by convective mass transport – resolution independent of flow
  • Ready to use columns
  • High-capacity for large molecules due to fully accessible void volume in the channels
  • Laminar flow minimizes shear stress resulting in high yields

Applications

  • Application
    Preparative Applications

Compliance Information

  • GMP Compliance
    No

Connections (Physical)

  • Connector(s)
    10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions

  • Channel Size
    2 µm
  • Column Tube Length
    56 mm
  • Inner Diameter
    6.5 mm
  • Outer Diameter
    15 mm

Features

  • Reusable
    Yes

General Specifications

  • Flow Rate
    0.8–100 mL/min | 2.4–335 cm/h | 0.1–12.5 CV/min

Materials of Construction

  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions

  • Maximum Operating Pressure
    2.0 MPa | 20 bar | 290 psi
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)
  • pH Stability
    Working range 2–13, cleaning in place 1–14

Physicochemical Information

  • Bed Volume
    8 mL
  • Ligand
    QA (strong anion exchanger, quaternary amine)
  • Ligand Density
    0.45–0.65 mmol/mL wet support
  • Typical Dynamic Binding Capacity 10% per mL
    ≥ 20 mg BSA/ml wet support, BSA, 3.0 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flow rate 16 mL/min

Product Information

  • Brand
    CIMmultus®
  • Package Type
    Individually Packed