CHO TF for SILAC, 1000 mL PET bottle
Perfect medium for working with transient CHO cells - growth, transfection and production -
CHO TF SILAC (without lysine and arginine) is a complete chemically defined, animal-component-free cell culture medium variant without arginine and lysine. Therefore, it can be used for SILAC (stable isotope labeling by/with amino acids in cell culture) experiments.
It was developed for cultivation of CHO and other mammalian cell lines, with a special focus on transfection applications and virus production. The medium is especially suited for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI). CHO TF SILAC supports cell growth and production of recombinant proteins and antibodies in high-density suspension culture. It can be used in research or in manufacturing applications.
- Item No.:
- 888-0001
- Min. Order Qty.
- 45
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CHO Media and Feeds
Suitable for various CHO clones (e.g., DG44, K1, CHO S, CHO GS)Chemically defined, serum-free, animal component-free and hydrolysate-freeLittle or no adaptation required from other serum-free mediaSupports stable growth of suspension cells at high viability in seed train cultureCompatibility with cationic transfection reagents (e.g. PEI) facilitates simplified transfectionw/o L-glutaminew/o growth factoravailable as "SILAC" variant w/o arginine and lysineApproved by ISO 9001 QMS (Quality Statement)
Applications
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ApplicationProtein
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Application AreaCHO cells
Compliance Information
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Animal Origin (AO) / Non-Animal Origin (NAO)NAO
Features
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Serum FreeYes
Physicochemical Information
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Adherence/SuspensionSuspension
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Chemically Defined (CD)Yes
Product Information
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Package TypeBottle
Storage Conditions
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Storage Conditions2 °C (36°F) to 8 °C (46°F); solution of ethanol and buffer (100 mM NaCl, 20 mM TRIS pH 7.5) in the ratio 1:4 (v/v)
Volume
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Volume1 L
Flexsafe® 2D bags, available in sizes from 20mL to 50L, allow for the storage, sampling, transfer, and shipping of all biopharmaceutical fluids in all process steps. Users can expect quality supply assurance, robustness, closure integrity, biocompatibility, and excellent cell growth performance.