Key Benefits
- Uses a DNA-specific dye so there is no need to use RNase.
- The dye crosses membranes so there is no need to permeabilize or fix the cells.
- This also means you can use live cells.
- No wash needed, simply add and read for fast, easily automated workflow.
Principle
Cells stained with the cell cycle dye will fluoresce in proportion to their DNA content. The cell cycle consists of four distinct phases: G0/G1 phase (growth), S phase (synthesis), G2 phase and M phase (mitosis). Each phase can be identified based on the relative amounts of DNA resident in the cell, with G0/G1 cells having the least amount of DNA and G2/M cells with the most. These relative differences in DNA content are easily detected by fluorescence intensity, and can be utilized to establish quantitative cell cycle profiles.
Multiplex with other iQue® kits like Cell Membrane Integrity for richer content.
