HEA HyperCel PRC Column - 1 mL

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HEA HyperCel PRC Column - 1 mL HEA HyperCel PRC Column - 1 mL
 

HEA HyperCel is a mixed mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH.

  • Pre-packed columns simplify resin screening and selection.
  • Ideal for process step optimization.
  • RoboColumn® format is especially suited for high throughput process development (HTPD).
Bed Volume
Price (CNY):
CNY892.26
Item No.: 
PRC05X050HEAHCEL
*Custom/bulk order quotes are provided within 72 hours of request.
HEA HyperCel PRC Column - 1 mL
HEA HyperCel PRC Column - 1 mL

HEA HyperCel is a mixed mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH.

  • Pre-packed columns simplify resin screening and selection.
  • Ideal for process step optimization.
  • RoboColumn® format is especially suited for high throughput process development (HTPD).
Price (CNY):
CNY892.26
Item No.:
PRC05X050HEAHCEL

Overview

Specifications

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HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

HEA HyperCel pre-packed columns are designed to simplify resin screening and selection, making them ideal for optimizing process steps. The RoboColumn® format is particularly suited for high throughput process development (HTPD), allowing for efficient and streamlined purification processes.

Features and Benefits

  • High mechanical and chemical stability
  • Suitable for large-scale chromatography in a biopharmaceutical environment
  • Cross-linked cellulose base matrix

Applications

  • Protein purification at low ionic strength by direct hydrophobic capture
  • Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
  • Orthogonal separation mechanism to ion exchange or HIC

Applications

  • Application
    Resin screening and process optimization

Compliance Information

  • Quality Standards
    • CoA
    • RSF (upon request)

Dimensions

  • Bead Size
    80 - 100 µm
  • Bed Height
    50 mm
  • Dimensions
    5 x 50 mm

General Specifications

  • Flow Rate
    100-300cm/h
  • Resin Format
    Pre-packed column

Materials of Construction

  • Base Matrix
    Rigid cellulose beads
  • Materials of Construction
    Polypropylene

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term), 2 to 12 (long term)

Physicochemical Information

  • Bed Volume
    1 mL
  • Ligand
    Hexylamine
  • Reference Protein
    Bovine serum albumin (BSA)
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA

Product Information

  • Brand
    HEA HyperCel
  • Pack Size
    1 column per pack
  • Pack Volume
    1 mL
  • Resin Delivery Condition
    24% iPrOH/100mM sodium phosphate, pH 4.3
  • Resin Type
    Mixed Mode anion exchanger
  • What you receive
    1 column, instructions

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl