MEP HyperCel is a flexible mixed mode chromatography resin designed for capture and purification of antibodies and various recombinant proteins from laboratory to manufacturing scale. MEP HyperCel resin offers a no salt/low salt alternative to conventional HIC and can simplify processes and improve economics by removing the need for salt addition and following UF/DF steps. The MEP ligand is immunoglobulin-selective. Thus, MEP Hypercel can be effectively used for IgG capture and for enhanced DNA, HCP and aggregate removal. Elution is under mild conditions.
MEP HyperCel pre-packed columns are designed to simplify resin screening and selection, making them ideal for optimizing process steps. The RoboColumn® format is particularly suited for high throughput process development (HTPD), allowing for efficient and streamlined purification processes.
Features and Benefits
- Unique separation mechanism and differentiated selectivity
- Direct protein capture from a variety of feedstocks
- No salt addition required for binding means a simpler process, lower costs and reduced waste
- Stable toward harsh cleaning with NaOH
Applications
- A no-salt/low-salt alternative to Hydrophobic Interaction Chromatography (HIC)
- Monoclonal and polyclonal IgG capture and intermediate purification (aggregate, DNA and HCP removal)
- Direct capture of recombinant proteins or enzymes from feedstock without need for pH or conductivity adjustments
