Vivaflow® 50, 100,000 MWCO PES, 2pc

100 ml - 3 l

  • Plug and play laboratory cross flow cassette
  • Modular - scalable to 6 units
  • Disposable
  • Optimal for aqueous sample concentration e.g. proteins and nanoparticles
  • High flow rate and recovery

Price (CNY):
CNY1,645.76
Item No.: 
VF05P4
Availability: 
Pieces
2

*Custom/bulk order quotes are provided within 24 hours of request.

Vivaflow® 50 is a disposable and ready-to-use crossflow cassette for filtration and concentration of samples from 100 ml to 3 l. The membrane surface per device is 50 cm2. One pack contains two devices and comes with all necessary accessories for running two devices with a laboratory pump and a size 16 pump head. For speeding up the concentration and increasing the volume throughput, six cassettes can be connected.

Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breeding facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 50 cm2 module typically reduces 500 ml to 15 ml in less than 50 min. Nearly total recovery of the concentrate is achieved with a single rinse. Up to six of the unique interlocking modules can be connected for easy scale up resulting in a capacity of several liters. Vivaflow® 50 is intended for single use. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Equipment required

The standard Vivaflow® 50 package comes with two cassettes, tubing, flow restrictor, one series interconnector and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Running more than two Vivaflow® 50 devices in parallel and series

For speeding up the concentration, as many as six Vivaflow® 50 cassettes can easily be connected using 2 T-connectors for connecting 2 Vivaflow® cassettes at the inlet and at the outlet and series interconnectors for connecting the devices inbetween. A pressure indicator needs to be built in the inlet for optimal control.

  • High flow rate and recovery
  • Clear housing for process monitoring
  • Ready to use single cassettes
  • Easy set up of multiple cassettes
  • Economical and disposable product
  • Unique in its performance

A single 50cm2 module will typically reduce 500 ml to less than 15 ml in under 50 minutes. Less than 10 ml minimum system recirculation for highest concentrations. Less than 1.5 ml non recoverable hold up volume. Near total recoveries achievable with a single 10 ml rinse.

Documents

Specifications

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性能参数

20°C,3 bar压力下,浓缩20倍

需要的时间 [分]

单一装置

三个装置

溶质回收率%

初始体积250 ml. min.

初始体积 1 L . min.

直接的

10 ml 漂洗

BSA 1.0 mg/ml (66,000 MW)

5,000 MWCO PES

34

49

96 %

99 %

10,000 MWCO PES

22

32

94 %

99 %

30,000 MWCO PES

22

32

92 %

99 %

50,000 MWCO PES

20

29

92 %

98 %

γ Globulins 1.0 mg/ml

100,000 MWCO PES

43

62

92 %

98 %

100,000 MWCO RC

40

58

92 %

98 %

Yeast 1.0 mg/ml (S.Cerevisiae)

0.2 µm PES

33<

47

92 %

98 %


Applications

  • Application
    Protein Concentration
  • Application Area
    Buffer exchange
  • Intended Use
    Research Use Only (RUO)
  • Market
    Research

Compliance Information

  • Animal Components
    No
  • CSA Compliant
    No
  • Certification
    None
  • Electrical / Battery item
    No

Connections (Physical)

  • Fittings
    Nylon (NY)

Dimensions

  • Channel Height
    0.3 mm
  • Channel Width
    15 mm
  • Membrane Area
    50 cm²
  • Overall Height
    84 mm
  • Overall Length
    107 mm

General Specifications

  • Pump Flow
    200 – 400 mL/min
  • Tubing
    PVC (medical grade)

Materials of Construction

  • Flow Channel
    TPX (PMP)
  • Flow Restrictor
    Polypropylene (PP)
  • Housing Material
    Polycarbonate (PC)
  • Material o-ring | seal
    Silicone (SI)
  • Membrane Material
    Polyethersulfone (PES)
  • Membrane Support
    TPX (PMP)
  • Pressure Indicator
    Polypropylene (PP), Stainless Steel (SS), Polyoxymethylene (POM), Silicone (SIL), Polyamide (PA)

Operating Conditions

  • Maximum Allowable Working Temperature
    60 °C (140 °F)
  • Maximum Operating Pressure
    3 bar (44 psi)

Physicochemical Information

  • MWCO
    100 kDa
  • Non-recoverable Hold-up
    < 0.5 mL
  • Pore Size
    N/A

Product Information

  • Brand
    Vivaflow®
  • Device Type
    TFF – (Crossflow)
  • Pack Size
    2
  • Sample Type
    • Protein
    • Peptide
    • Nanoparticles
    • Virus
    • Endotoxin

Volume

  • Dead Stop Volume
    N/A
  • Hold-up Volume
    1.5 mL
  • Minimum Recirculation Volume
    10 mL
  • Volume Range
    100 – 3,000 mL

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