Vivacon® 500, 2,000 MWCO Hydrosart, 25pc

For up to 500 µl

Vivacon® 500 are centrifugal units for fast DNA concentration and buffer exchange of 500 µl samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart® membranes results in high recoveries from dilute samples. Complete recovery of the concentrate is guaranteed by a reverse spinning step.

Price (CNY):
CNY1,148.71
Item No.: 
VN01H91
Availability: 
Pieces
25

*Custom/bulk order quotes are provided within 24 hours of request.

Vivacon® 500 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 500 µl initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 500 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.

High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 500 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.

用于DNA的性能特点

初始体积 2 ml, 样品浓度50 ng/ml

样品大小 (bp)

20℃浓缩至30X 时间(分)

浓缩回收率 %

加压离心 (xg)

2,000 MWCO

10

120 min.

92 %

7,500

10,000 MWCO

30

60 min.

94 %

5,000

30,000 MWCO

50

60 min.

95 %

2,500

50,000 MWCO

300

45 min.

96 %

2,500

100,000 MWCO

600

30 min.

93 %

2,500

125,000 MWCO

650

30 min.

88 %

2,500

125,000 MWCO

900

30 min.

89 %

2,500

用于蛋白质的性能特点

初始体积 2 ml, 蛋白浓度如表中标注

样品

20°C,浓缩30倍需要的时间 [分] 

浓缩回收率 %

离心力

2,000 MWCO

0.25 mg/ml cytochrome c

120 min.

95 %

7,500

10,000 MWCO

0.25 mg/ml cytochrome c

90 min.

96 %

5,000

30,000 MWCO

1.0 mg/ml BSA

40 min.

96 %

5,000

50,000 MWCO

1.0 mg/ml BSA

30 min.

94 %

5,000

100,000 MWCO

1.0 mg/ml bovine IgG

30 min.

92 %

5,000

125,000 MWCO

1.0 mg/ml bovine IgG

27 min.

81 %

5,000


Applications

  • Application
    • Protein Concentration  
    • DNA Concentration  
  • Application Area
    • Buffer exchange  
    • Diagnostic sample prep  
  • Market
    • BioPharma  
    • Research  
    • Forensic  

Compliance Information

  • Animal Components
    No  
  • CSA Compliant
    No  
  • Certification
    None  
  • Electrical / Battery item
    No  

Features

  • PCR Grade
    No  

Materials of Construction

  • Concentrator Body/Sleeve
    Polycarbonate (PC)  
  • Filtrate Vessel
    Polypropylene (PP)  
  • Membrane Material
    Hydrosart® (HY)  

Physicochemical Information

  • MWCO
    2 kDa  

Product Information

  • Brand
    Vivacon®  
  • Device Type
    Centrifugal  
  • Pack Size
    25  
  • Sample Type
    • DNA  
    • Peptide  

Volume

  • Dead Stop Volume
    5 µL  
  • Maximum Capacity, Fixed Angle Rotor
    500 µL  
  • Minimum Recirculation Volume
    N/A  
  • Volume Range
    0.1 – 0.5 mL  

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