Blue Trisacryl® M is an affinity resin that is used for the purification of enzymes, interferons, and some coagulation factors, as well as for the removal of albumin. The resin uses a Trisacryl base matrix which carries the blue dye ligand.

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Blue Trisacryl® M
Blue Trisacryl® M

Blue Trisacryl® M is an affinity resin that is used for the purification of enzymes, interferons, and some coagulation factors, as well as for the removal of albumin. The resin uses a Trisacryl base matrix which carries the blue dye ligand.

Item No.:
M_Blue_Trisacryl
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Overview

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Blue Trisacryl® M resin is a dye-affinity chromatographic resin used for the purification of a wide variety of enzymes and proteins such as kinases, albumin, interferons, and some coagulation factors. The basic matrix is the macroporous non-ionic Trisacryl support on which Blue dye has been covalently immobilized.

Features and Benefits

  • Unique multi-modal interaction mechanism
  • Low ligand leakage
  • Multiple applications from laboratory to production scale

Applications

  • Enzymes which need NAD as cofactor (kinases, dehydrogenases, phosphatases)
  • Other enzymes (sulfatases, RNA polymerases, mono-oxygenases, oxidoreductases)
  • Albumin from plasma
  • Transferin from plasma
  • α1-acid glycoprotein, α-fetoprotein, α1-proteinase inhibitor
  • Serine proteases Interferons
  • DNA antibodies
  • Recombinant vaccines purification processes

Applications

  • Application
    Preparative protein purification

Compliance Information

  • Quality Standards
    • CoA
    • RSF

Dimensions

  • Bead Size
    40-80 µm

General Specifications

  • Flow Rate
    100-150 cm/h
  • Resin Format
    Bulk resin

Materials of Construction

  • Base Matrix
    Synthetic Polymer

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 12 (short term), 4 to 10 (long term)

Physicochemical Information

  • Ligand
    Blue dye
  • Reference Protein
    HSA
  • Typical Dynamic Binding Capacity 10% per mL
    >10 mg/mL HSA

Product Information

  • Brand
    Blue Trisacryl® M
  • Pack Size
    1
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Affinity resin
  • What you receive
    • 1 bottle resin
    • Instruction of use

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl