CIMmultus PrimaS® 8000 mL cGMP Compliant Monolithic Column (2 μm)
This multimodal column combines anion exchange and hydrogen bonding properties, making its selectivity entirely distinct from traditional anion exchangers.
It is most often employed for the capture of RNA in crude samples such as IVT mixtures.
- Item No.:
- 931.5118-2
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMmultus®
CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.
They can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
The ligand in PrimaS® contains a series of hydrogen donors and hydrogen acceptors, combined with a weak anion exchange character. Its hydrogen bonding ability makes its selectivity entirely distinct from traditional anion exchangers. PrimaS purifies large single-stranded mRNA (ssRNA) under aqueous conditions at ambient temperature. Contaminants are removed using a high salt wash, while ssRNA is recovered from the column with a pH gradient. It can be used for purification of ssRNA, including RNA exceeding 10 kb (such as self-amplifying RNA). In a purification process, it is most often employed for the capture of RNA in crude samples such as IVT mixtures.
PrimaS features:
- Multimodal chromatography ligand that combines elements of hydrogen bonding with anion exchange chromatography
- Offers unique selectivities in many important product areas
- Supports purification of single-stranded RNA at ambient temperature
- Fractionates ssRNA by size
Your benefits:
- Very fast processes by convective mass transport – resolution independent of flow
- Ready to use columns
- High-capacity for large molecules due to fully accessible void volume in the channels
- Laminar flow minimizes shear stress resulting in high yields
Applications
-
ApplicationPreparative Applications
Compliance Information
-
GMP ComplianceYes
Connections (Physical)
-
Connector(s)TC 1" (25 mm), 8 mm ID bore
Dimensions
-
Channel Size2 µm
-
Column Tube Length410
-
Inner Diameter255 mm
-
Outer Diameter300 mm
Features
-
ReusableYes
General Specifications
-
Flow Rate800–8000 mL/min | 13–135 cm/h | 0.1–1 CV/min
Materials of Construction
-
Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
-
Maximum Operating Pressure0.7 MPa | 7 bar | 100 psi
-
Recommended Operating Temperature4 °C (39 °F) to 40 °C (104 °F)
-
pH StabilityWorking range 2–13, cleaning in place 1–14
Physicochemical Information
-
Bed Volume8000 mL
-
LigandMultimodal (anion exchange-hydrogen bonding)
Product Information
-
BrandCIMmultus®
-
Package TypeIndividually Packed