CIMmultus® is a unique and industry-proven line of pre-packed chromatographic monolithic columns featuring large flow-through channels, designed for working with large biomolecules such as viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), nucleic acids (mRNA, pDNA), virus-like particles and exosomes. CIMmultus® columns can be used as single-use disposable or multi-use columns. They are available in a variety of volume ranges – from 1mL for screening and method development purposes to 8000mL for industrial manufacturing.
CIMmultus® PrimaT is a monolithic chromatographic multimodal column primarily used for purification of adeno-associated virus (AAV) to separate AAV sub-species. Separation is based on weak anion exchange, H-bond and metal affinity coordination interactions with the AAV. CIMmultus® PrimaT allows binding of all AAV serotypes and provides advanced removal of product-related impurities and AAV sub-species, while maintaining high recovery rates.
Main benefits of CIMmultus® PrimaT:
- AAV binding at neutral or near neutral pH: Multimodal properties of CIMmultus® PrimaT allow binding of AAV at neutral or near-neutral pH values.
- Enables separation of different AAV subpopulations: Unique multimodal elution separation mechanism usually leads to separation of full AAV from different product-related impurities.
- Endotoxin Removal: Allows at least 5 log of endotoxin removal.
Due to the multimodal properties of CIMmultus® PrimaT column, elution based on different concentrations of metal ions is recommended, such as starting with an ascending linear MgCl2 gradient. Characteristics of binding and elution interactions between AAV and CIMmultus® PrimaT vary for each specific AAV capture eluate sample, primarily dependent on the purity of capture step eluate and AAV serotype.
