Heparin HyperD® M

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Heparin HyperD® M
 

Heparin HyperD® M resin is suited for the preparative-scale purification of heparin binding proteins like coagulation factors, growth hormones, lipoproteins, and DNA/RNA processing enzymes.

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Heparin HyperD® M
Heparin HyperD® M

Heparin HyperD® M resin is suited for the preparative-scale purification of heparin binding proteins like coagulation factors, growth hormones, lipoproteins, and DNA/RNA processing enzymes.

Item No.:
M_Heparin_HyperD

Overview

Specifications

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Heparin HyperD® M "gel-in-a-shell" resin is comprised of a porous rigid mineral bead containing hydrogel filled pores. The heparin ligand is bound to the hydrogel.

Features and Benefits

  • High dynamic binding capacity at high linear flow rate
  • Rigid, non-compressible resin
  • Purification of various proteins from laboratory to production scale
  • Minimal ligand leakage

Applications

  • Reference method for large-scale purification of antithrombin III (ATIII)
  • Purification of coagulation factors such as Factor IX, Factor VII, Factor XI, Factor XII and XIIa
  • Lipoprotein(LDL, VLDL, VLDL apoprotein, HDL) and lipoprotein lipase purification
  • Growth hormone and growth factor (FGF, ECGF) purification
  • DNA- and RNA-related enzymes
  • Purification of various other enzymes (collagenase, α-L-iduronidase, hyaluronidase and lysozyme), fibronectin, fibronectin fragments and hormones receptors

Applications

  • Application
    Preparative protein purification

Compliance Information

  • Quality Standards
    • CoA
    • RSF

Dimensions

  • Bead Size
    80 µm

General Specifications

  • Flow Rate
    100-600 cm/h
  • Resin Format
    Bulk resin

Materials of Construction

  • Base Matrix
    Mineral beads

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    3 to 13 (working pH)

Physicochemical Information

  • Ligand
    Porcine heparin
  • Reference Protein
    ATIII
  • Typical Dynamic Binding Capacity 10% per mL
    25UI/mL ATIII

Product Information

  • Brand
    Heparin HyperD® M
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Affinity resin

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl