MEP HyperCel provides a unique separation mechanism and selectivity for protein separations, with ligand-based immunoglobulin selectivity optimal for monoclonal antibody capture and intermediate purification. The MEP ligand facilitates the binding of immunoglobulins as well as other target proteins at neutral pH under moderate salt concentrations.

  • Pre-packed columns simplify resin screening and selection
  • Ideal for process step optimization
  • RoboColumn® format is especially suited for high throughput process development (HTPD)
Bed Volume
Price (CNY):
CNY892.26
Item No.: 
PRC05X050MEPHCEL
*Custom/bulk order quotes are provided within 72 hours of request.
MEP HyperCel PRC Column - 1 mL
MEP HyperCel PRC Column - 1 mL

MEP HyperCel provides a unique separation mechanism and selectivity for protein separations, with ligand-based immunoglobulin selectivity optimal for monoclonal antibody capture and intermediate purification. The MEP ligand facilitates the binding of immunoglobulins as well as other target proteins at neutral pH under moderate salt concentrations.

  • Pre-packed columns simplify resin screening and selection
  • Ideal for process step optimization
  • RoboColumn® format is especially suited for high throughput process development (HTPD)
Price (CNY):
CNY892.26
Item No.:
PRC05X050MEPHCEL

Overview

Specifications

MEP HyperCel is a flexible mixed mode chromatography resin designed for capture and purification of antibodies and various recombinant proteins from laboratory to manufacturing scale. MEP HyperCel resin offers a no salt/low salt alternative to conventional HIC and can simplify processes and improve economics by removing the need for salt addition and following UF/DF steps. The MEP ligand is immunoglobulin-selective. Thus, MEP Hypercel can be effectively used for IgG capture and for enhanced DNA, HCP and aggregate removal. Elution is under mild conditions.

MEP HyperCel pre-packed columns are designed to simplify resin screening and selection, making them ideal for optimizing process steps. The RoboColumn® format is particularly suited for high throughput process development (HTPD), allowing for efficient and streamlined purification processes.

Features and Benefits

  • Unique separation mechanism and differentiated selectivity
  • Direct protein capture from a variety of feedstocks
  • No salt addition required for binding means a simpler process, lower costs and reduced waste
  • Stable toward harsh cleaning with NaOH

Applications

  • A no-salt/low-salt alternative to Hydrophobic Interaction Chromatography (HIC)
  • Monoclonal and polyclonal IgG capture and intermediate purification (aggregate, DNA and HCP removal)
  • Direct capture of recombinant proteins or enzymes from feedstock without need for pH or conductivity adjustments

Applications

  • Application
    Resin screening and process optimization

Compliance Information

  • Quality Standards
    • CoA
    • RSF

Dimensions

  • Bead Size
    80 - 100 µm
  • Bed Height
    50 mm
  • Dimensions
    5 x 50 mm

General Specifications

  • Flow Rate
    100-300cm/h
  • Resin Format
    Pre-packed column

Materials of Construction

  • Base Matrix
    Rigid cellulose beads
  • Materials of Construction
    Polypropylene

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    2 to 14 (short term), 2 to 12 (long term)

Physicochemical Information

  • Bed Volume
    1 mL
  • Ligand
    4-mercaptoethylpyridine
  • Reference Protein
    Human polyclonal IgG
  • Typical Dynamic Binding Capacity 10% per mL
    ≥ 20 mg/mL hIgG at 6' residence time

Product Information

  • Brand
    MEP HyperCel
  • Pack Size
    1 column per pack
  • Pack Volume
    1 mL
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed Mode anion exchanger
  • What you receive
    1 column, instructions

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl