The Octet® Kinetics Buffer 10X is necessary for kinetics assays. Kinetics Buffer solutions are required at various steps throughout the assay process. Having enough buffer on hand is critical for ensuring your kinetics assays continue without interruption or issue.
Maintaining pH Levels
Maintaining the appropriate, desired pH level during complex, multi-step kinetics assays is essential for good data quality and results. Best results are obtained when all matrices are matched as closely as possible across multiple assay steps.
Use Throughout Kinetics Assay
Buffer solution, like the Kinetics Buffer 10X, is perhaps the most common and widely used solution in kinetics experiments, necessary for dilution, optimization and other purposes. It is used in pre-hydration, hydration, biosensor priming, loading and multiple steps from baseline, association and dissociation. It is crucial during regeneration (i.e., cycling regeneration buffer and kinetics buffer during regeneration steps).
Compatibility with Octet® Platforms and Biosensors
The Kinetics Buffer solution is essential for kinetics assays on the Octet® platform and is compatible with all Octet® biosensors.
Diluting to 1X Kinetics Buffer
Several biosensors and kinetics assays require a more diluted solution of Kinetics Buffer. Often, 1X Kinetics Buffer is recommended. It should be prepared by diluting the Octet® Kinetics Buffer 10X 10-fold, with 1X PBS, pH 7.4.
Use Buffer as Reference Sample
Octet® Kinetics Buffer 10X can be used as a reference sample when dealing with subtraction of baseline drift. A reference sample is a buffer-only control with no analyte present. Using a reference sample in the association step can help correct for baseline downward drift.
