Octet® SPR HisCap sensor chips consist of pre-immobilized nitrilotriacetic acid (NTA) within a nondextran polysaccharide 3D surface, which can be used for affinity capture coupling. In comparison to the strong interaction between streptavidin and biotin, the lower bond strength observed between NTA and a His-tag allows easy removal of His-tagged ligand proteins and regeneration of sensor chip surfaces using a variety of conditions, including imidazole, SDS and EDTA.
Top Features:
High binding capacity
Popular Applications:
Kinetics
Affinity
Screening
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