Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling

Incucyte^® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling was formulated explicitly for cell labeling of living mammalian cells. The dye is a cell-permeable DNA strain that specifically stains nuclei in cells and is ideally suited to the mix-and-read, real-time quantitation. A simple three step process allows for the ability to dye, seed and image your samples for straightforward quantification and analysis.

Addition of the Incucyte^® Nuclight Rapid Red Dye to normal healthy cells is non-perturbing to cell growth and morphology and provides homogenous staining of nuclei. When added to tissue culture medium, the inert stain crosses the cell membrane and has excellent specificity for DNA without the need for a wash step. With the Incucyte^® integrated analysis software, fluorescent objects can be quantified and background fluorescence minimized. This reagent has been validated for use with the Incucyte^® Live-Cell Analysis System and enables the real-time quantification of cell proliferation. Furthermore, our Incucyte^® Nuclight Rapid Red Dye can be combined with Incucyte^® Cell Health Reagents and Incucyte^® Confluence Metrics to quantify cell proliferation alongside apoptosis, cytotoxicity, or viability in a single well.

Presentation, Storage and Stability

Incucyte^® Nuclight Rapid Red Dye is supplied as a single vial (50 µL) in dimethylsulfoxide (DMSO), with each vial providing sufficient quantity for performing 100–200 tests (1 test = 1 well of a 96-well microtiter plate). Upon receipt, the solution should be stored at 4° C. When stored as described, the stock solutions will be stable for at least 12 months.

Recommended Use

We recommend optimizing Incucyte^® Nuclight Rapid Red Dye for each cell line tested by diluting the reagent in growth media (final dilutions of 1:250, 1:500, 1:1000, 1:2000 and 1:4000) and adding directly to cells in culture. Immediately post addition, cells are labeled with the Nuclight Rapid Red Dye. When used in an Incucyte^® Live-Cell Analysis System, we recommend data collection every 2–3 hours.

Additional protocol details can be found in the Product Guide.