Vivacon® 500, 10,000 MWCO Hydrosart, 25pc
For up to 500 µl
Vivacon® 500 are centrifugal units for fast DNA concentration and buffer exchange of 500 µl samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart® membranes results in high recoveries from dilute samples. Complete recovery of the concentrate is guaranteed by a reverse spinning step.
- Price (CNY):
- CNY1,152.75
- Item No.:
- VN01H01
- Availability:
- pcs
- Pieces
- 25
*Custom/bulk order quotes are provided within 24 hours of request.
Choose your Vivacon® 500 Centrifugal Units
Vivacon® 500 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 500 µl initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 500 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.
High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 500 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
初始体积 2 ml, 样品浓度50 ng/ml 样品大小 (bp) 20℃浓缩至30X 时间(分) 浓缩回收率 % 加压离心 (xg) 2,000 MWCO 10 120 min. 92 % 7,500 10,000 MWCO 30 60 min. 94 % 5,000 30,000 MWCO 50 60 min. 95 % 2,500 50,000 MWCO 300 45 min. 96 % 2,500 100,000 MWCO 600 30 min. 93 % 2,500 125,000 MWCO 650 30 min. 88 % 2,500 125,000 MWCO 900 30 min. 89 % 2,500 初始体积 2 ml, 蛋白浓度如表中标注 样品 20°C,浓缩30倍需要的时间 [分] 浓缩回收率 % 离心力 2,000 MWCO 0.25 mg/ml cytochrome c 120 min. 95 % 7,500 10,000 MWCO 0.25 mg/ml cytochrome c 90 min. 96 % 5,000 30,000 MWCO 1.0 mg/ml BSA 40 min. 96 % 5,000 50,000 MWCO 1.0 mg/ml BSA 30 min. 94 % 5,000 100,000 MWCO 1.0 mg/ml bovine IgG 30 min. 92 % 5,000 125,000 MWCO 1.0 mg/ml bovine IgG 27 min. 81 % 5,000用于DNA的性能特点
用于蛋白质的性能特点
Application Notes
Brochure
Catalog
Flyer
Poster
Applications
-
Application
-
Protein Concentration
- DNA Concentration
-
Protein Concentration
-
Application Area
-
Buffer exchange
- Diagnostic sample prep
-
Buffer exchange
-
Market
-
BioPharma
-
Research
- Forensic
-
BioPharma
Compliance Information
-
Animal ComponentsNo
-
CSA CompliantNo
-
CertificationNone
-
Electrical / Battery itemNo
Features
-
PCR GradeNo
Materials of Construction
-
Concentrator Body/SleevePolycarbonate (PC)
-
Filtrate VesselPolypropylene (PP)
-
Membrane MaterialHydrosart® (HY)
Physicochemical Information
-
MWCO10 kDa
Product Information
-
BrandVivacon®
-
Device TypeCentrifugal
-
Pack Size25
-
Sample Type
-
DNA
- Peptide
-
DNA
Volume
-
Dead Stop Volume5 µL
-
Maximum Capacity, Fixed Angle Rotor500 µL
-
Minimum Recirculation VolumeN/A
-
Volume Range0.1 – 0.5 mL
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