The Lysis Reactor is a single-use component to use in combination with the In-line Lysis System’s Tower. Together, they form a fully automated in-line lysis system with a closed single-use loop for process development (PD) up to pilot scale. The Lysis Reactor is a single-use component with simple installation, requires little countertop space and provides a clean and simple alternative to larger batch lysis processes, allowing users to use it as a plug-and-play combination. It consists of the main reactor body with a fixed volume of 5000 mL, 5 inlet paths (4 solutions and one air) and an outlet path.