Choose your Sartobind® Rapid A
Sartobind® Rapid A Cassettes integrate Sartorius agarose-based membrane functionalized with Protein A ligand in a stackable module. Using the Pilot Filter Holder, up to 13 cassettes can be connected, for a bed volume of up to 10.4 L. Despite the different formats, their performance is directly scalable to the capsule formats. Sartobind® Rapid A is engineered for time and cost-efficient mAb purification, offering competitive binding capacity (typically between 40-50 g/L1) and low pressure drop. With a simple and quick set-up and working residence times as low as 10 seconds, the overall processing time and costs can be significantly decreased. Sartobind® Rapid A Cassettes are well-suited for earlier clinical-phase and low-demand commercial manufacturing.
Features and Benefits:
- Bed volume: 0.8 L/cassette
- Bed-height: 4 mm
- Max. operating pressure: 2 bar
- Directly scalability to capsule formats
- Typical flow rates: 5 – 10 MV/min2
Applications:
- mAb purification
- Clinical stage and lower demand commercial manufacturing
Operational Requirements:
- Cassette Holder: Hydraulic clamping system for assembly and connection of the Sartobind® Rapid A cassettes.
- Manifold Set: Includes the inlet and outlet plates for the Sartobind® Rapid A cassettes operation as well as the venting and draining tubing.
1dependent on the target molecule.; 2for more information please consult Sartobind® Rapid A Instructions for Use
Application Notes
Applications
-
ApplicationCapture
Compliance Information
-
Biological Reactivity
-
The final product meets the requirements of the current ISO 10993-5 which is equivalent to the current United States Pharmacopoeia (USP) <87> Biological Reactivity Tests, in vitro.
- All in-contact plastic components meet the requirements of current USP <88> Biological Reactivity Tests, in vivo, for Plastic Class VI (Systemic Injection, Intracutaneous and Implantation Tests).
-
The final product meets the requirements of the current ISO 10993-5 which is equivalent to the current United States Pharmacopoeia (USP) <87> Biological Reactivity Tests, in vitro.
Connections (Physical)
-
Connector(s)Sanitary 1" – 1.5"
Dimensions
-
Bed Height4 mm
-
Dimensions634 × 49 × 387 mm
Materials of Construction
-
Housing MaterialAcrylonitrile butadiene styrene (ABS)
Operating Conditions
-
Maximum Operating Pressure0.2 MPa
-
Maximum Venting Pressure0.5 bar, 0.05 MPa, 7 psi
-
Recommended Flow Rate3-5 MV/min (load, elution), 5-10 MV/min ( equilibration, wash)
-
pH StabilitypH 2 – 14 (short term), pH 3 – 13 (long term)
Physicochemical Information
-
Bed Volume0.8 L
-
Chemical CompatibilityStable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
-
LigandProtein A
-
Reference ProteinHuman polyclonal IgG
-
Typical Dynamic Binding Capacity 10% per mL≥ 30 mg/mL
-
Void Volume2500 mL
Product Information
-
BrandSartobind® Rapid A
-
Membrane Adsorber FormatCassettes
-
Membrane TypeAffinity
-
Pack Size1
-
What you receive
-
1 cassette
-
1 operating instruction
- 1 certificate
-
1 cassette