The Sartobind® Rapid A Ligand is a recombinant Protein A expressed in Escherichia coli to be used as a reference material for ELISA-based quantification of leached Protein A in eluate fractions of Sartobind® Rapid A. It streamlines quantification, ensures consistent results, and is easy to solubilize and use with clear instructions.

Item No.: 
ZL-R-PAQ10
Piece
1
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Sartobind® Rapid A Ligand
Sartobind® Rapid A Ligand

The Sartobind® Rapid A Ligand is a recombinant Protein A expressed in Escherichia coli to be used as a reference material for ELISA-based quantification of leached Protein A in eluate fractions of Sartobind® Rapid A. It streamlines quantification, ensures consistent results, and is easy to solubilize and use with clear instructions.

Item No.:
ZL-R-PAQ10
Number of pieces
1

Overview

Specifications

Sartobind® Rapid A Ligand is a high-purity recombinant Protein A expressed in Escherichia coli, specifically designed for ELISA-based quantification of leached Protein A in eluate fractions. This ligand is optimized for use with the Repligen ELISA Kit 9000-1, facilitating accurate analysis of Protein A leachables after chromatographic purification. Each vial contains 10 mg of high-purity recombinant Protein A, as lyophilized powder, ready for immediate use after solubilization.

Key Features:

  • High Purity: Provides reliable and consistent quantification of leached Protein A.
  • Efficiency: Streamlines the process of quantifying Protein A leachables, saving valuable time in the lab.
  • Easy Solubilization: Lyophilized powder is ready for immediate use with clear instructions.
  • ELISA Optimization: Specifically qualified for compatibility with Repligen ELISA Kit 9000-1.

Applications:

  • Leached Protein A quantification
  • Quality Control

Applications

  • Application
    • Analytics
    • mAb Purification

Compliance Information

  • Biological Reactivity
    The final product meets the requirements of the current ISO 10993-5 which is equivalent to the current United States Pharmacopoeia (USP) <87> Biological Reactivity Tests, in vitro.

Dimensions

  • Bed Height
    4 mm

Operating Conditions

  • Recommended Flow Rate
    3-5 MV/min (load, elution), 5-10 MV/min (equilibration, wash)
  • pH Stability
    pH 2-14 (short term), pH 3-13 (long term)

Physicochemical Information

  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Ligand
    Protein A
  • Reference Protein
    Human polyclonal IgG
  • Typical Dynamic Binding Capacity 10% per mL
    > 38 mg/mL

Product Information

  • Brand
    Sartobind® Rapid A
  • Membrane Type
    Affinity
  • Pack Size
    1