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CIMac SO3 0.1 mL Analytical Column (1.3 µm)
CIMac SO3 0.1 mL Analytical Column (1.3 µm)
This high-performance ion-exchange column is primarily intended for fast, efficient and reproducible analysis and in-process control of samples throughout different purification steps.
Overview
Specifications
Choose your CIMac
CIMac Analytical Columns are intended for fast and reproducible HPLC monitoring and quantitation of large proteins, plasmid DNA, viruses, virus-like particles, and phages. They were primarily designed for the in-process and final control of various samples at different stages of the purification process. They can be operated at high volumetric flow rates (from 1 to 20 column volumes/min); therefore, the information about the product quantity and purity can be obtained within a few minutes. CIMac Analytical Columns are fast and reliable PAT tools, providing:
- Rapid, high resolution analysis, achieved in minutes
- Flow independent separation
- High sensitivity and reproducibility
SO3 (Sulfonyl) features:
- Strong cation exchanger
- Working range, pH 2–13
- Binds molecules with predominantly positive charge, repels molecules a predominantly negative charge
Applications
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ApplicationAnalytical Columns
Connections (Physical)
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Connector(s)10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
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Channel Size1.3 µm
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Diameter5.2 mm
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Length4.95 mm
Features
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ReusableYes
General Specifications
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Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
Materials of Construction
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Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure15 MPa | 150 bar | 2175 psi
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Recommended Operating Temperature2 °C (36 °F) to 25 °C (77 °F)
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pH StabilityWorking range 2–13, cleaning in place 1–14
Physicochemical Information
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Bed Volume0.1 mL
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LigandSO3 (strong cation exchanger, sulfonate)
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Typical Dynamic Binding Capacity 10% per mL25-31 mg Lysozyme/mL wet support, Lysozyme 0.5 mg/mL, 20 mM phosphate buffer,pH 7.5, flow rate 1 mL/min
Product Information
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BrandCIMac™